Molecular cloning and sequencing of the chicken smooth muscle myosin regulatory light chain

FEBS Letters

Kendrick-Jones, John¹  Messer, Neil G.¹ 

[1] MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, England

关键词: Myosin light chain;    cDNA cloning;    Nucleotide sequence;    (Chicken gizzard);    bp;    base pair;    Tris;    tris(hydroxymethyl)aminomethane;   

DOI  :  10.1016/0014-5793(88)81300-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA probe was constructed from a chicken skeletal muscle regulatory light chain cDNA and was used to screen a chicken gizzard cDNA library. A clone containing the entire coding region of the chicken gizzard regulatory light chain was isolated and sequenced. The deduced protein sequence is identical to the most recently reported chemical sequence of the chicken smooth muscle regulatory light chain, and has homologies with other troponin C-like calcium-binding proteins.

【 授权许可】

Unknown