FEBS Letters | |
Inverse gene expression of prostacyclin and thromboxane synthases in resident and activated peritoneal macrophages | |
Inoue, Hiroyasu2  Tanabe, Tadashi2  Tone, Yoshinori2  Kuwamoto, Shinsuke2  Izumi, Yoshikazu1  | |
[1] Department of Biotechnology, Faculty of Engineering, University of Tottori, Tottori 680, Japan;Department of Pharmacology, National Cardiovascular Center Research Institute, Suita, Osaka 565, Japan | |
关键词: Prostacyclin synthase; Thromboxane synthase; Cyclooxygenase; Prostaglandin; Peritoneal macrophage; PG; prostaglandin; PGI2; prostacyclin; TX; thromboxane; PGIS; prostacyclin synthase; TXS; thromboxane synthase; BCG; bacillus Calmette-Guérin; PES; prostaglandin endoperoxide synthase; bp; base pair(s); kb; kilobase pair(s); PCR; polymerase chain reaction; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; | |
DOI : 10.1016/S0014-5793(97)00516-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
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【 摘 要 】
Prostacyclin and thromboxane A2 produced from prostaglandin H2 are known to be important modulators with opposite biological activities. To examine possible roles of these prostanoids in immune responses, we have studied the gene expression of prostacyclin synthase (PGIS) and thromboxane synthase (TXS) in murine resident macrophages or in macrophages elicited with casein or bacillus Calmette-Guérin (BCG). Northern blot analyses showed that the PGIS mRNA was expressed in a decreasing order in the resident, and casein- and BCG-elicited macrophages. In contrast, the TXS mRNA was expressed in an increasing order in the resident, and casein- and BCG-elicited macrophages. On the other hand, the mRNA for cyclooxygenase-2, which produces PGH2 and participates in the production of prostanoids in inflammation, was expressed in both the resident and BCG-elicited macrophages but barely in the casein-elicited cells. In situ hybridization analysis showed that the expression of mRNAs for PGIS and TXS was ascribable not only to the alteration of the expression levels of both mRNAs in the each macrophage but also to the changes in subpopulations of the cells expressing these mRNAs. These observations suggested that the inverse gene expression of PGIS and TXS in macrophages contributes to immune responses by modulating the relative levels of prostacyclin and thromboxane A2.
【 授权许可】
Unknown
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