Potential role of group X secretory phospholipase A2 in cyclooxygenase‐2‐dependent PGE2 formation during colon tumorigenesis

FEBS Letters

Morioka, Yasuhide¹  Hanasaki, Kohji¹  Saiga, Akihiko¹  Fujii, Noriko¹  Ikeda, Minoru¹  Arita, Hitoshi¹  Ishimoto, Yoshikazu¹ 

[1] Shionogi Research Laboratories, Shionogi and Co., Ltd., Sagisu 5-12-4, Fukushima-ku, Osaka 553-0002, Japan

关键词: Colon tumor;    Phospholipase A2;    Secretory phospholipase A2;    Cytosolic phospholipase A2;    Cyclooxygenase;    Prostaglandin;    NSAIDs;    non-steroidal anti-inflammatory drugs;    COX;    cyclooxygenase;    FAP;    familial adenomatous polyposis;    PG;    prostaglandin;    PLA2;    phospholipase A2;    cPLA2;    cytosolic PLA2;    sPLA2;    secretory PLA2;    sPLA2-IB;    group IB sPLA2;    sPLA2-IIA;    group IIA sPLA2;    sPLA2-X;    group X sPLA2;    BSA;    bovine serum albumin;    PBS;    phosphate-buffered saline;    PPARδ;    peroxisome proliferator-activated receptor δ;   

DOI  :  10.1016/S0014-5793(00)02350-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Although the cyclooxygenase-2 (COX-2) pathway of the arachidonic acid cascade has been suggested to play an important role in colon carcinogenesis, there is little information concerning the identity of phospholipase A₂ (PLA₂) involved in the arachidonic acid release in colon tumors. Here, we compared the potencies of three types of secretory PLA₂s (group IB, IIA and X sPLA₂s) for the arachidonic acid release from cultured human colon adenocarcinoma cells, and found that group X sPLA₂ has the most powerful potency in the release of arachidonic acid leading to COX-2-dependent prostaglandin E₂ (PGE₂) formation. Furthermore, immunohistological analysis revealed the elevated expression of group X sPLA₂ in human colon adenocarcinoma neoplastic cells in concert with augmented expression of COX-2. These findings suggest a critical role of group X sPLA₂ in the PGE₂ biosynthesis during colon tumorigenesis.

【 授权许可】

Unknown