Identification of group X secretory phospholipase A2 as a natural ligand for mouse phospholipase A2 receptor

FEBS Letters

Higashino, Ken-ichi¹  Hanasaki, Kohji¹  Arita, Hitoshi¹  Nakano, Kazumi¹  Yokota, Yasunori¹ 

[1] Shionogi Research Laboratories, Shionogi and Co. Ltd., 12-4 Sagisu, 5-Chome, Fukushima-ku, Osaka 553-0002, Japan

关键词: Phospholipase A2;    Secretory phospholipase A2;    Group X secretory phospholipase A2;    Phospholipase A2 receptor;    Lipid mediator;    PLA2;    phospholipase A2;    sPLA2;    secretory phospholipase A2;    sPLA2-IB;    group IB sPLA2;    PLA2R;    phospholipase A2 receptor;    sPLA2-IIA;    group IIA sPLA2;    sPLA2-X;    group X sPLA2;    DSS;    disuccinidyl suberate;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(00)01848-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phospholipase A₂ receptor (PLA₂R) mediates various biological responses elicited by group IB secretory phospholipase A₂ (sPLA₂-IB). The recently cloned group X sPLA₂ (sPLA₂-X) possesses several structural features characteristic of sPLA₂-IB. Here, we detected a specific binding site of sPLA₂-X in mouse osteoblastic MC3T3-E₁ cells. Cross-linking experiments demonstrated its molecular weight (180 kDa) to be similar to that of PLA₂R. In fact, sPLA₂-X was found to bind the recombinant PLA₂R expressed in COS-7 cells, and its specific binding detected in mouse lung membranes was abolished by the deficiency of PLA₂R. These findings demonstrate sPLA₂-X to be one of the high-affinity ligands for mouse PLA₂R.

【 授权许可】

Unknown