Clearance of group X secretory phospholipase A2 via mouse phospholipase A2 receptor

FEBS Letters

Notoya, Mitsuru¹  Higashino, Ken-ichi¹  Hanasaki, Kohji¹  Arita, Hitoshi¹  Nakano, Kazumi¹  Ishimoto, Yoshikazu¹  Yokota, Yasunori¹ 

[1] Shionogi Research Laboratories, Shionogi and Co., Ltd., Sagisu 5-12-4, Fukushima-ku, Osaka 553-0002, Japan

关键词: Secretory phospholipase A2;    Group X secretory phospholipase A2;    Phospholipase A2 receptor;    Internalization;    Clearance receptor;    PLA2;    phospholipase A2;    sPLA2;    secretory phospholipase A2;    sPLA2-X;    group X sPLA2;    sPLA2-IB;    group IB sPLA2;    sPLA2-IIA;    group IIA sPLA2;    COX;    cyclooxygenase;    PG;    prostaglandin;    PLA2R;    phospholipase A2 receptor;    Ab;    antibody;    CHO;    Chinese hamster ovary;    MEM;    minimum essential medium;    FCS;    fetal calf serum;    PBS;    phosphate-buffered saline;    BSA;    bovine serum albumin;    LAMP-2;    lysosome-associated membrane protein type 2;    IgG;    immunoglobulin G;   

DOI  :  10.1016/S0014-5793(01)03173-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Given the potent hydrolyzing activity toward phosphatidylcholine, group X secretory phospholipase A₂ (sPLA₂-X) elicits a marked release of arachidonic acid linked to the potent production of lipid mediators in various cell types. We have recently shown that sPLA₂-X can also act as a ligand for mouse phospholipase A₂ receptor (PLA₂R). Here, we found that sPLA₂-X was internalized and degraded via binding to PLA₂R associated with the diminished prostaglandin E₂ (PGE₂) formation in PLA₂R-expressing Chinese hamster ovary (CHO) cells compared to CHO cells. Indirect immunocytochemical analysis revealed that internalized sPLA₂-X was co-localized with PLA₂R in the punctate structures in PLA₂R-expressing CHO cells. Moreover, in mouse osteoblastic MC3T3-E₁ cells that endogenously express the PLA₂R, the internalized sPLA₂-X was localized in lysosomes. These findings demonstrate that PLA₂R acts as a clearance receptor for sPLA₂-X to suppress its strong enzymatic activity.

【 授权许可】

Unknown