Expression of mRNA for cyclooxygenase‐1 and cyclooxygenase‐2 in human tissues

FEBS Letters

Ford-Hutchinson, Anthony W.¹  O'Neill, Gary P.¹ 

[1] Department of Pharmacology, Merck Frosst Centre for Therapeutic Research, Merck Frosst Canada Inc., PO Box 1005, Pointe Claire-Dorval, Que. H9R 4P8, Canada

关键词: Cyclooxygenase;    Prostaglandin synthase;    Prostaglandin;    Thromboxane;   

DOI  :  10.1016/0014-5793(93)80263-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rate-limiting step in the formation of prostanoids is the conversion of arachidonic acid to prostaglandin H₂ by cyclooxygenase, also known as prostaglandin G/H synthase/cyclooxygenase. Two forms of cyclooxygenase have been characterized: a ubiquitously expressed form (COX-1) and a recently described second form (COX-2) inducible by various factors including mitogens, hormones, serum and cytokines. Here we quantitate by the reverse transcriptase-polymerase chain reaction (RT-PCR) the expression of COX-1 and COX-2 mRNA in human tissues including lung, uterus, testis, brain, pancreas, kidney, liver, thymus, prostate, mammary gland, stomach and small intestine. All tissues examined contained both COX-1 and COX-2 mRNA and could be grouped according to the level of COX mRNA expression. The highest levels of COX mRNAs were detected in the prostate where approximately equal levels of COX-1 and COX-2 transcripts were present. In the lung high levels of COX-2 were observed whereas COX-1 mRNA levels were about 2-fold lower. An intermediate level of expression of both COX-1 and COX-2 mRNA was observed in the mammary gland, stomach, small intestine, and uterus. The lowest levels of COX-1 and COX-2 mRNA were observed in the testis, pancreas, kidney, liver, thymus, and brain.

【 授权许可】

Unknown