Phorbol ester‐induced secretion of human hepatocyte growth factor by human skin fibroblasts and its inhibition by dexamethasone

FEBS Letters

Yamamoto, Itaru¹  Tsubouchi, Hirohito²  Gohda, Eiichi¹  Kataoka, Hirotoshi¹  Daikilara, Yasushi³ 

[1] Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Okayama 777, Japan;Second Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Kagoshima 890, Japan;Department of Biochemistry, Kagoshima University Dental School, Kagoshima 890, Japan

关键词: Human hepatocyte growth factor;    Phorbol ester;    Protein kinase C;    Human skin fibroblast;    Dexamethasone;    DNA synthesis;    hHGF;    human hepatocyte growth factor;    HGF;    hepatocyte growth factor;    PMA;    phorbol 12-myristate 13-acetate;    PDBu;    phorbol 12.13-dibutyrate;    4α-PDD;    4α-phorbol 12;    13-didecanoate;    PKC;    protein kinase C;    hSF;    human scatter factor;    MEM;    (Eagle's) minimum essential medium;    ELISA;    enzyme-linked immunosorbent assay;    BSA;    bovine serum albumin;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(92)80220-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human skin fibroblasts secreted a certain amount of human hepatocyte growth factor (hHGF), as determined by an enzyme-linked immunosorbent assay for hHGF. This hHGF secretion was remarkably stimulated by protein kinase C (PKC)-activating phorbol esters, which was inhibited by the simultaneous addition of dexamethasone. Pretreatment with phorbol 12-myristate 13-acetate (PMA) caused a down-regulation in hHGF secretion. hHGF secreted by the PMA-treated cells showed a potent hepatocyle growth-promoting activity which was neutralized by an anti-hHGF antiserum. These results indicate both that PMA-treated human skin fibroblasts produce biologically active hHGF and the possible involvement of PKC activation in this process.

【 授权许可】

Unknown