Phospholipase D treatment enhances gonadotrophin receptor‐coupled adenylate cyclase activity in isolated bovine luteal cells

FEBS Letters

Budnik, Lygia T.¹  Mukhopadhyay, Amal K.¹ 

[1] Institute for Hormone and Fertility Research, University of Hamburg, Hamburg, Germany

关键词: Phosphatidic acid;    Cyclic AMP;    Phorbol ester;    Protein kinase C;    Signal transduction;   

DOI  :  10.1016/0014-5793(93)81795-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

LH-stimulated adenylate cyclase activity in membrane preparations of bovine luteal cells could be enhanced by treating the cells with either phospholipase D or its hydrolysis product, phosphatidic acid. Similar augmentary effects were also produced following treatment of the cells with EGF. Moreover, EGF could stimulate the formation of [³H]phosphatidic acid in [³H]myristic acid preloaded cells, suggesting that EGF is able to activate cellular phospholipase D. Also, PMA was able to increase the phosphatidic acid formation with a parallel increase in the adenylate cyclase activity. We propose, therefore, that phosphatidic acid may act as an intracellular second messenger linking EGF-mediated activation of phospholipase D with the sensitization of LH receptor-coupled adenylate cyclase signalling system.

【 授权许可】

Unknown