Epinephrine and the Ca2+ ionophore A23187 synergistically induce platelet aggregation without protein kinase C activation

FEBS Letters

Olbrich, Christine¹  Siess, Wolfgang¹ 

[1] Medizinische Klinik Innenstadt der Universität München, Ziemssenstrasse 1, D8 München 2, FRG

关键词: Ca2+ ionophore;    Epinephrine;    Synergism;    Platelet aggregation;    Protein kinase C;    Phosphatidic acid;   

DOI  :  10.1016/0014-5793(89)80144-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Aspirin-pretreated, ³²P-prelabeled, washed human platelets resuspended in a buffer containing apyrase and 2% plasma were exposed to epinephrine and the Ca²⁺ ionophore A23187. Epinephrine potentiated platelet aggregation (not secretion), the production of [³²P]phosphatidic acid and myosin light chain phosphorylation induced by A23187. No phosphorylation of the 40 kDa protein, the substrate of protein kinase C, was observed. We conclude that G₁-protein activation evoked by epinephrine and Ca²⁺ mobilization caused by A23187 represents a novel synergism for platelet aggregation and that protein kinase C activation, under these conditions is not needed for platelet aggregation.

【 授权许可】

Unknown