Histamine release by pharmacological agents in the absence of external free Ca2+

FEBS Letters

Carrasco, M.¹  Pintado, E.¹  Goberna, R.¹ 

[1] Department of Biochemistry, University of Seville Medical School, Avda. Sánchez Pizjuán, 4 Sevilla 9, Spain

关键词: Ca2+ ionophore;    Compound;    Exocytosis;    Histamine secretion;    Mast cell;    Potassium depolarization;   

DOI  :  10.1016/0014-5793(84)80588-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The involvement of extracellular free Ca²⁺ in histamine release was investigated in rat peritoneal mast cells. Incubation of non-antigenized cells in a media with high extracellular potassium did not increase histamine release. Secretion induced by A23187 and compound in the presence of Ca²⁺ requires metabolic energy. In the absence of external free Ca²⁺ (2.5 μM) histamine release induced by A23187 is reduced but not abolished. Secretion induced by compound is independent of extracellular Ca²⁺. These results lead us to suggest that mast cell plasma membranes probably lack voltage-gated Ca²⁺ channels and that external Ca²⁺ may not be an absolute requisite for histamine secretion.

【 授权许可】

Unknown