Estimation of adenosine triphosphate utilization of rat mast cells during and after anaphylactic histamine secretion

FEBS Letters

Johansen, Torben¹ 

[1] Institute of Medical Biology, Department of Pharmacology, The Medical School, Odense University, J.B. Winsløws Vej 19, DK-5000 Odense C, Denmark

关键词: Mast cell;    Adenosine triphosphate;    Anaphylaxis;    Histamine secretion;    Exocytosis;   

DOI  :  10.1016/0014-5793(90)80983-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Determination of the cellular content of adenosine triphosphate (ATP) and the rate of ATP-synthesis were used to estimate the cellular utilization ofATP in relation to anaphylactic histamine secretion. There was an increased rate of oxidative ATP-synthesis and a decreased cellular ATP content during the time period of histamine secretion and immediately after its completion. During secretion the additional ATP-utilization above the basal level of ATP-synthesis was 0.51 cells. 2.5 min after cell activation, the rate of additional ATP-utilization was 0.30 , and the persistent ATP-decrease observed after 30 and 120 min may be due to a decreased rate of oxidative ATP-synthesis.

【 授权许可】

Unknown