期刊论文详细信息
The Japanese Journal of Pharmacology
Purinoceptor-Mediated Calcium Mobilization and Cellular Proliferation in Cultured Bovine Corneal Endothelial Cells
Kweon-Haeng Lee1  Takashi Sekine2  Hitoshi Endou2  Tae-Won Hahn3  Seok Ho Cha2 
[1] Department of Pharmacology,Catholic University Medical College,505 Banpo-dong,Seoul 137-701,Korea;Department of Pharmacology and Toxicology,Kyorin University School of Medicine,Mitaka,Tokyo 181-8611,Japan;Department of Ophthalmology,Catholic University Medical College,Kangnam St.Mary’s Hospital,505 Banpo-dong,Seocho-gu,Seoul 137-701,Korea
关键词: Corneal endothelium;    Adenosine triphosphate;    Cytosolic free calcium;    Proliferation;   
DOI  :  10.1254/jjp.82.181
学科分类:药理学
来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society
PDF
【 摘 要 】

References(29)Cited-By(15)In the present study, we investigated the effect of adenosine triphosphate(ATP)on cytosolic free calcium mobilization and mitogenic activity in cultured bovine corneal endothelial cells(BCEC).The[Ca2+]i was determined using a Ca2+ sensitive indicator, Fura-2/AM, and cell proliferation was evaluated by counting the cell number.ATP, its metabolites and analogs caused transient increase in[Ca2+]i in a concentration-dependent manner(10-7M−10-3M)and the potency of agonists was ordered as follows:2-methylthio-ATP>uridine triphosphate>ATP>adenosine diphosphate.Adenosine monophosphate and adenosine did not affect[Ca2+]i.ATP(10-4M)also promoted the accumulation of inositol trisphosphate(IP3).The ATP-induced transient[Ca2+]i increase and IP3 accumulation were attenuated by pretreatment with a phospholipase C inhibitor, U-73122(5μM), for 30 min.ATP(10-5M)significantly enhanced the proliferation of BCEC.ATP-induced[Ca2+]i increase and cell proliferation were inhibited by a purinoceptor antagonist, suramin(10-4M).Thus, the present study indicates that BCEC contain P2 purinoceptors that regulate their proliferation.

【 授权许可】

Unknown   

【 预 览 】
附件列表
Files Size Format View
RO201912080714633ZK.pdf 488KB PDF download
  文献评价指标  
  下载次数:6次 浏览次数:26次