Ca2+ and phorbol ester synergistically induce HL‐60 differentiation

FEBS Letters

Tyers, Michael¹  Harley, Calvin B.¹ 

[1] Department of Biochemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada

关键词: Synergism;    Phorbolester;    Ca2+ ionophore;    Differentiation;    (HL-60 cell);    DMSO;    dimethyl .sulfoxide;    OAG;    1-oleoyl-2-acetylglycerol;    PDBu;    phorbol 12;    13-dibutyrate;    PDD;    phorbol 12;    13-didecanoate;    4α-PDD;    4α'-phorbol 12;    13-didecanoate;    TPA;    12-O-tetrade-canoylphorbol 13-acetate;   

DOI  :  10.1016/0014-5793(86)81348-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure of HL-60 cells to subthreshold concentrations of TPA caused monocytic differentiation only when cells were cotreated with the Ca²⁺ ionophore A23187. Phorbol ester dose-response curves for growth arrest and enzymatic markers of differentiation were shifted to lower concentrations when the ionophore was present. Expression of a monocyte/granulocyte cell surface antigen also occurred only when cells were treated with both agents. Similar effects were seen with other active but not inactive phorbol esters and with another Ca²⁺ ionophore. The Ca²⁺ component of phosphoinositide-based signalling may thus play a role in HL-60 differentiation.

【 授权许可】

Unknown