Phorbol esters induce nitric oxide synthase and increase arginine influx in cultured peritoneal macrophages

FEBS Letters

Boscá, Lisardo¹  Hortelano, Sonsoles¹  Genaro, Ana M.¹ 

[1] Instituto de Bioquímica, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain

关键词: Nitric oxide;    Phorbol ester;    Protein kinase C;    Macrophage;    1;    2-DOG;    sn-1;    2-dioctanoylglycerol;    EGF;    epidermal growth factor;    β-TGF;    transforming growth factor β;    INF-γ;    interferon-γ;    d-NMA;    N G-methyl-d-arginine;    l-NMA;    N G-methyl-l-arginine;    l-NMA;    N ω-nitro-l-arginine;    LPS;    lipopolysaccharide;    NOS;    nitric oxide synthase;    PDBu;    β-phorbol 12;    13-dibutyrate;    α-PDD;    α-phorbol 12;    13-didecanoate;    PMA;    β-phorbol 12-myristate;    13-acetate;   

DOI  :  10.1016/0014-5793(93)80078-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of peritoneal macrophages with β-phorbol 12,13-dibutyrate promotes a time-dependent release of NO to the incubation medium. This effect was antagonized by LPS, a well known inducer of nitric oxide synthase (NOS) expression in macrophages, and was inhibited by N ^G-methyl-l-arginine and N ^ω-nitro-l-arginine. An increase in intracellular cGMP and NOS activity was observed in parallel with NO release. The induction of NOS was accompanied by a stimulation of arginine influx within the cell. These results suggest that activation of protein kinase C by phorbol esters is sufficient to promote NOS induction in macrophages.

【 授权许可】

Unknown