Nuclear localization of protein kinase Cα and its association with nuclear components in Neuro‐2a neuroblastoma cells

FEBS Letters

Hucho, Ferdinand¹  Buchner, Klaus¹  Lindschau, Carsten² 

[1] Institute for Biochemistry, Free University of Berlin, Thielallee 63, 14195 Berlin, Germany;Franz Vollhard Clinic, Max Delbrück Centre for Molecular Medicine, 13122 Berlin-Buch, Germany

关键词: Protein kinase C;    Protein kinase Cα;    Nucleus;    Nucleoplasm;    Confocal microscopy;    Phorbol ester;   

DOI  :  10.1016/S0014-5793(97)00226-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using activity measurements and Western blotting, we demonstrated that PKCα is constitutively present in nuclei of Neuro-2a neuroblastoma cells. Confocal microscopy revealed that PKCα is present in the nucleoplasm and that this localization does not change after stimulation with phorbol ester. However, as revealed by extraction experiments, phorbol ester leads to a firmer association of PKCα with nuclear components. Our findings suggest that PKCα not only associates with lipids but also with proteins inside the nucleus. The presence of active PKCα inside the nucleus allows the enzyme to phosphorylate not only proteins at the nuclear envelope but also proteins in the nucleoplasm.

【 授权许可】

Unknown