NPC 15437 interacts with the C1 domain of protein kinase C An analysis using mutant PKC constructs

FEBS Letters

Connor, Jane R.¹  Sullivan, James P.¹  Tiffany, Carol¹  Burch, Ronald M.¹  Shearer, Barry G.¹ 

[1] Nova Pharmaceutical Corporation, 6200 Freeport Centre, Baltimore, MD 21224, USA

关键词: Protein kinase C;    NPC 15437;    Inhibitor;    Phorbol ester;   

DOI  :  10.1016/0014-5793(91)80739-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently demonstrated that 2,6,diamino-N-([I-(oxotridecyl)-2-piperidinyl]methyl)-hexanamide (NPC 15437) is a selective inhibitor of PKC interacting at the regulatory domain of the enzyme. To further investigate the interaction of NPC 15437 with PKC we expressed a series of cDNAs encoding mutant PKC molecules in COS7 cells. NPC 15437 had no effect on the protein kinase activity of mutants lacking the N-terminal region of the C1 domain. Further, NPC 15437 was a competitive inhibitor of the activation of PKCα by phorbol ester and attenuated the binding of phorbol ester to the enzyme in intact cells. The present study demonstrates that mutant enzyme constructs can be used to localize the site of interaction of NPC 15437 with PKC to residues 12–42, which encodes the pseudosubstrate binding domain and part of the first cysteine-rich repeat sequence.

【 授权许可】

Unknown