Heat‐shock responsive elements in the induction of the multidrug resistance gene (MDR1)

FEBS Letters

Kioka, Noriyuki¹  Komano, Tohru¹  Ueda, Kazumitsu¹  Yamano, Yoshiaki¹ 

[1] Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University, Kyoto 606, Japan

关键词: Multidrug resistance;    MDR1;    P-Glycoprotein;    Heat-shock protein;    HSE;    heat-shock responsive element;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    CAT;    chloramphenicol acetyltransferase;   

DOI  :  10.1016/0014-5793(92)80205-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The MDR1 gene, considered to be involved in multidrug resistance of cancer cells, is expressed in liver, kidney, small intestine and the blood-brain barrier. We investigated MDR1 gene expression in the well-differentiated hepatoma cell line HepG2 after exposure to several stresses and found that sodium arsenite treatment increased MDR1 gene expression 2.6-fold. Deletion analysis of the MDR1 promoter indicated that the transcriptional activation after exposure to arsenite depends on a 60-bp region containing two heat-shock responsive elements.

【 授权许可】

Unknown