A theoretical method to predict DNA permutation gel electrophoresis from the sequence

FEBS Letters

Boffelli, D.¹  Palleschi, A.¹  Risuleo, G.²  Savino, M.²  De Santis, P.¹ 

[1] Dipartimento di Chimica, Università di Roma ‘La Sapienza’, P. le A. Moro 5, 00185 Roma, Italy;Dipartimento di Genetica e Biologia Molecolare, Università di Roma ‘La Sapienza’ P. le A. Moro 5, 00185 Roma, Italy

关键词: Theoretical prediction;    DNA curvature;    DNA permutation;    Gel electrophoresis;   

DOI  :  10.1016/0014-5793(92)80190-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gel electrophoretic permutation assays of DNA fragments experimentally investigated by different authors were theoretically reproduced using our theoretical model of sequence-dependent curvature. The general pattern of agreement obtained suggests that our method can be usefully adopted as an alternative to the experimental assay, in particular where the lack of a sufficient number of unique restriction sites in the fragment prevents the correct localization of the main bend site.

【 授权许可】

Unknown