Evaluation of cell sheet application on one wall bone defect in Macaca nemestrina through periostin expression

1st Physics and Technologies in Medicine and Dentistry Symposium

物理学;医药卫生

Tamin, R.Y.^1 ; Soeroso, Y.^1 ; Amir, L.^2 ; Idrus, E.^2

Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia^1

Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia^2

关键词: After-treatment;    Gel electrophoresis;    Gingival crevicular fluids;    Healthy bones;    Oral disease;    Periodontal tissue;    Protein bands;    Tissue repair;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/884/1/012039/pdf
DOI  :  10.1088/1742-6596/884/1/012039

学科分类：卫生学

来源: IOP

【 摘 要 】

Chronic periodontitis is an oral disease in which the destruction of periodontal tissue leads to tooth loss. Regenerative therapy for attachment cannot be applied to one wall bone defects owing to the minimal existing healthy bone. Tissue engineering in the form of cell sheets has been developed to overcome this limitation. In a previous study, cell sheet application to a one wall bone defect in Macaca nemestrina showed good clinical results. To evaluate the effectiveness of cell sheet application histologically, the level of periostin expression in the gingival crevicular fluid (GCF) of M. nemestrina was determined. Periostin is a 90-kDa protein that regulates coordination and interaction for regeneration and tissue repair. A laboratory observation study was performed to see the differences in periostin levels in samples collected from M. nemestrina's GCF, where a cell sheet was applied to the bone defect. Gel electrophoresis with SDS-PAGE was performed to detect periostin expression based on its molecular weight and to compare the expression band between the cell sheet and the control at 1, 2, and 3 weeks after treatment. The gel electrophoresis result shows different thicknesses of the protein band around the molecular weight of periostin between the cell sheet groups.