Analysis of nucleoid-associated protein-binding regions reveals DNA structural features influencing genome organization in Mycobacterium tuberculosis

FEBS Letters

article

Sharmilee Sarkar¹  Upalabdha Dey¹  Trust Boitumelo Khohliwe¹  Venkata Rajesh Yella²  Aditya Kumar¹ 

[1] Department of Molecular Biology and Biotechnology, Tezpur University;Department of Biotechnology, Koneru Lakshmaiah Education Foundation

关键词: DNA curvature;    DNA flexibility;    DNA shape;    genome condensation;    Mycobacterium tuberculosis;    NAPs;   

DOI  :  10.1002/1873-3468.14178

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nucleoid-associated proteins (NAPs) maintain bacterial nucleoid configuration through their architectural properties of DNA bending, wrapping, and bridging. However, the contribution of DNA structural alterations to DNA-NAP recognition at the genomic scale remains unresolved. Present work dissects the DNA sequence, shape and altered structural preferences at a genomic scale for six NAPs in Mycobacterium tuberculosis . Results suggest narrower minor groove width (MGW) and higher DNA rigidity are marked for the binding sites of EspR and Lsr2, while mIHF, MtHU and NapM have heterogeneous DNA structural predilections. In contrast, WhiB4–DNA-binding sites were characterized by wider MGW, highly deformable and less curved DNA. This work provides systematic insight into NAP-mediated genome organization as a function of DNA structural features.

【 授权许可】

Unknown