Endothelin stimulates both cAMP formation and phosphatidylinositol hydrolysis in cultured embryonic bovine tracheal cells

FEBS Letters

Watakabe, Tadashi¹  Okuda-Ashitaka, Emiko²  Fujitani, Yasushi¹  Inui, Takashi¹  Okada, Toshikazu¹  Ito, Seiji²  Urade, Yoshihiro¹  Oda, Kyoko¹ 

[1] International Research Laboratories, Ciba-Geigy (Japan) Ltd., 10-66 Miyuki-cho, Takarazuka 665, Japan;Department of Cell Biology, Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka 565, Japan

关键词: Endothelin;    Receptor;    cAMP;    Phosphatidylinositol turnover;    EBTr cell;    embryonic bovine tracheal cells;    ET;    endothelin;    PI;    phosphatidylinositol;    IBMX;    3-isobutyl-l-methylxanthine;    TPA;    12-O-tetradecanoylphorbol 13-acetate;    PG;    prostaglandin;    DMEM;    Dulbecco's modified Eagle's medium;    HEPES;    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonicacid;    HBSS;    HEPES-buffered salt solution;    [Ca2+]i;    intracellular Ca2+ concentration;    IP;    inositol phosphate;    IPi;    inositol monophosphate;    IP2;    inositol bisphosphate;    IP3;    inositol trisphosphate;   

DOI  :  10.1016/0014-5793(92)80244-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Embryonic bovine tracheal (EBTr) cells were found to possess receptors for endothelin (ET) of ET-1-selective (ET_A)subtype with a K _d for ET-1 of 114 pM and a B _max of 12.9 fmol/10⁵ cells. Stimulation of EBTr cells with 100 pM to 100 nM ET-1 increased the contents of both inositol phosphates and cAMP in a concentration-dependent manner, indicating that the receptors are coupled to both phosphatidylinositol hydrolysis and cAMP formation in EBTr cells.

【 授权许可】

Unknown