Contraction of smooth muscle by activation of endothelin receptors on autonomic neurons

FEBS Letters

Inui, Takashi¹  Okada, Toshikazu¹  Urade, Yoshihiro¹  Takimoto, Misato¹ 

[1] International Research Laboratories, Ciba-Geigy Japan Limited, 10-66 Miyuki-cho, Takarazuka 665, Japan

关键词: Endothelin;    Receptor;    Autonomic neuron;    Smooth muscle;    Contraction;    ET;    endothelin;    DMEM;    Dulbecco's modified Eagle's medium;    HEPES;    N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid;    [Ca2+]i;    intracellular Ca2+ concentration;   

DOI  :  10.1016/0014-5793(93)80134-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Endothelin receptors, predominantly of the ET_B type, were localized to cell bodies, processes, and varicosities of cholinergic and adrenergic intramural autonomic neurons that were present in primary cultures of guinea pig tracheal smooth muscle. Stimulation of the neuronal ET_B receptor produced a tetrodotoxin-sensitive increase in the intracellular calcium concentration in neurons which was followed by contraction of the neighboring smooth muscle cells. These observations suggest that endothelins can induce smooth muscle contraction by means of a neuronally mediated mechanism, in addition to their direct actions on the smooth muscle.

【 授权许可】

Unknown