Thromboxane receptor stimulation inhibits adenylate cyclase and reduces cyclic AMP‐mediated inhibition of ADP‐evoked responses in fura‐2‐loaded human platelets

FEBS Letters

Heemskerk, Johan W.M.¹  Sage, Stewart O.¹ 

[1] The Physiological Laboratory, University of Cambridge, Downingstreet, Cambridge, CB2 3EG, UK

关键词: Platelet;    Thromboxane A2;    Adenylate cyclase;    Calcium;    Manganese;    Fluorescent indicator;    [Ca2+];    cytosolic calcium concentrations;    cAMP;    3′;    5′-cyclic AMP;    PG;    prostaglandin;    TxA2;    thromboxane A2;   

DOI  :  10.1016/0014-5793(92)80056-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stimulation of human platelets with the thromboxane A₂ analogue, U46619, after treatment with prostaglandin E₁ or forskolin, reduced the inhibition of ADP-evoked Mn²⁺ influx and the release of Ca²⁺ from intracellular stores, U46619 decreased the elevated concentration of 3′, 5′-cyclic AMP in platelets that were pretreated with prostaglandin E₁. These results suggest that occupation of prostaglandin H₂/thromboxane A₂ receptors. like those for other agonists, inhibits adenylate cyclase activity, which can contribute to the promotion of platelet activation.

【 授权许可】

Unknown