Regulation of [3H]forskolm binding to human platelet membranes by GppNHp, NaF, and prostaglandin E1

FEBS Letters

Nelson, Carol A.¹  Seamon, Kenneth B.¹ 

[1] Division of Biochemistry and Biophysics, Food and Drug Administration, Bethesda, MD 20205, USA

关键词: Forskolin;    Adenylate cyclase;    Platelet;    Receptor;    Binding;    Cyclic AMP;    GppNHp;    guanosine 5 '-β;    γ-imidodiphosphate;    [3H]forskolin;    [12-3H]forskolin;    Ns;    stimulatory guanine nucleotide regulatory protein;   

DOI  :  10.1016/0014-5793(85)80808-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Displaceable binding of [³H]forskolin to human platelet membranes can be detected in the presence of magnesium. There is an increase in the number of [³H]forskolin binding sites when membranes are incubated with GppNHp or NaF in the presence of magnesium. Prostaglandin E₁, which stimulates human platelet adenylate cyclase, does not affect the binding of [³H]forskolin in the absence of GppNHp. However, the dose-response curve for the GppNHp-dependent increase in [³H]forskolin binding sites is shifted to lower concentrations in the presence of prostaglandin E₁. Prostaglandin E₁ potentiates the effect of GppNHp on [³H]forskolin binding most likely by facilitating the binding of the guanine nucleotide at the stimulatory quanine nucleotide regulatory protein of adenylate cyclase.

【 授权许可】

Unknown