Inhibition of Forskolin‐stimulated cardiac adenylate cyclase activity by short‐chain alcohols

FEBS Letters

Chatelain, Pierre¹  Waelbroeck, Magali¹  Jean, Christophe¹  Jean-Claude, Camus¹  Robberecht, Patrick¹ 

[1] Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Bld. de Waterloo 115, B-1000 Brussels, Belgium

关键词: Adenylate cyclase;    Forskolin;    Short-chain alcohol;    Ethanol;    Rat heart;    Gpp(NH)p;    guanosine 5′-O-(2-3-imido)-triphosphate;   

DOI  :  10.1016/0014-5793(83)80904-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The diterpene forskolin stimulated rat cardiac adenylate cyclase activity at least 20-fold and potentiated the effect of NaF. The stimulatory effect of forskolin was reduced in the presence of Gpp(NH)p. Ethanol markedly reduced the stimulation of adenylate cyclase by forskolin while potentiating NaF and Gpp(NH)p stimulation. The inhibitory effect of ethanol on forskolin stimulation appeared to be of a mixed type with both a competitive and a non-competitive component. Three other short-chain linear alcohols (methanol, propanol, butanol) also inhibited forskolin-stimulation, this effect being proportional to the number of carbon atoms.

【 授权许可】

Unknown