Construction and characterization of a fusion protein with epidermal growth factor and the cell‐binding domain of fibronectin

FEBS Letters

Taguchi, Yuki¹  Ohdate, Yoichi¹  Kato, Ikunoshin¹  Kimizuka, Fusao²  Shimojo, Tomoko¹  Kawase, Yasutoshi¹ 

[1] Biotechnology Research Laboratories, Takara Shuzo Co. Ltd., Seta 3-4-1, Otsu, Shiga 520-21, Japan;Bioproducts Development Center, Takara Shuzo Co. Ltd., Sunaike 2257, Noji, Kasatsu, Shiga 525, Japan

关键词: Fibronectin;    Epidermal growth factor;    Fusion protein;    Recombinant plasmid;    Drug delivery system;    FN;    fibronectin;    EGF;    apidermal growth factor;    DDS;    drug delivery system;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(92)80037-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An efficient expression system was constructed for C-EGF, a fusion protein made of a fragment of the cell-binding domain of human fibronectin (FN) bound with epidermal growth factor (EGF), C-EGF was produced in Escherichia coli HB101 cells carrying the recombinant plasmid pCE102 as inclusion bodies, which were solubilized and refolded after purification. C-EGF had both cell-adhesive and EGF activities, so it might be more effective than EGF in therapeutic applications. This fusion system would be useful for the construction of a recombinant drug delivery system for cells that have fibronectin receptors (integrins).

【 授权许可】

Unknown