Localization of the cellular‐fibronectin‐specific epitope recognized by the monoclonal antibody IST‐9 using fusion proteins expressed in E. coli

FEBS Letters

Baralle, Francisco E.²  Zardi, Luciano¹  Carnemolla, Barbara¹  Owens, Raymond J.²  Borsi, Laura¹ 

[1] Cell Biology Laboratory, Istituto Nazionale per la Ricerca sub Cancro, Viale Benedetto XV, 10, 16132 Genova, Italy;Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, England

关键词: Fibronectin;    Monoclonal antibody;    Fusion protein;    Splicing;   

DOI  :  10.1016/0014-5793(87)80160-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we report on a monoclonal antibody (IST-9) which distinguishes between human cellular and plasma fibronectin. Using β-galactosidase-fibronectin fusion proteins expressed in E. coli we have demonstrated that this monoclonal antibody is specific for a fibronectin segment (ED) which can be included or omitted from the molecule depending on the pattern of splicing of the mRNA precursors. Furthermore, using the same fusion proteins we have been able to localize precisely the epitopes of two other monoclonal antibodies (IST-1 and IST-2), specific for the heparin-binding domain 5 of fibronectin.

【 授权许可】

Unknown