Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin

FEBS Letters

Cartwright, A.J.²  Bloomfield, J.F.¹  Morris, G.E.²  Man, Nguyen thi²  Love, D.R.¹  Davies, K.E.¹ 

[1] Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, UK;Research Division, N.E. Wales Institute, Deeside, Clwyd, CH5 4BR, U.K.

关键词: Dystrophin;    Muscular dystrophy;    Monoclonal antibody;    Epitope mapping;    Fusion protein;    Nitrothiocyanolsenzoic acid;   

DOI  :  10.1016/0014-5793(90)80199-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nineteen monoclonal antibodies which bind to native dystrophin in the plasma membrane of frozen muscle sections were obtained using a recombinant fusion protein as immunogen. On Western blots of normal mouse muscle extracts, the antibodies bind specifically to a 400 000 M _r protein which is absent from dystrophic mouse (mdx) muscle. At least four distinct epitopes have been identified by cleavage mapping methods. Although the fusion protein contained 25% of the human dystrophin sequence (Cys⁸¹⁶-Asp¹⁷⁴⁷; M _r 108 000), most of the monoclonal antibodies (15 out of 19) recognize a single fragment of M _r 27 500.

【 授权许可】

Unknown