Monoclonal antibodies targeted against the C‐terminal domain of dystrophin or utrophin

FEBS Letters

Léger, Jocelyne¹  Fabbrizio, Eric¹  Mornet, Dominique¹  Anoal, Monique¹  Léger, Jean J.¹ 

[1] INSERM U 300, Physiopathologie Cellulaire et Moleculaire, Bâtiment K, Faculté de Pharmacie, Avenue Charles Flahault 34060, Montpellier, France

关键词: Dystrophin;    Utrophin;    Monoclonal antibody;    Epitope mapping;    Recombinant protein;    DMD;    Duchenne muscular dystrophy;    ELISA;    enzyme-linked immunosorbent assay;    SDS;    sodium dodecyl sulfate;    EDTA;    ethylene diamine tetraacetic acid;    EGTA;    ethylene glycoether tetraacetic acid;    PMSF;    phenyl methyl sulfonyl fluoride;   

DOI  :  10.1016/0014-5793(93)81100-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The structure-function relationships of dystrophin, a protein which is absent or defective in patients with Duchenne or Becker muscular dystrophies, and utrophin can only be compared if specific antibodies are produced. We expressed C-terminal parts of dystrophin and utrophin in expression vectors. Mice were immunized with recombinant proteins and 26 monoclonal antibodies were produced and analyzed. Their respective epitopes were determined using other overlapping recombinant products. We observed antibody specificity towards 400 kDa dystrophin and/or utrophin protein bands, either by Western blot analysis or immunodetection in human skeletal (quadriceps) and smooth (uterus) muscles. These antibodies have been used to compare the relative abundance of both dystrophin and utrophin relative to the structures analyzed.

【 授权许可】

Unknown