Monoclonal antibody evidence for structural similarities between the central rod regions of actinin and dystrophin

FEBS Letters

Cartwright, A.J.²  Moorman, A.F.M.¹  Morris, G.E.²  Ginjaar, I.B.³  Ellis, J.M.²  Man, Nguyen thi²  van Ommen, G.-J.B.³  van Paassen, M.M.B.³ 

[1] Anatomisch-Embryologisch Laboratorium, Academisch-Medisch Centrum, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands;Research Division, NE Wales Institute, Deeside, Clwyd CH4 5BR, UK;Department of Human Genetics, Sylvius Laboratories, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands

关键词: Dystrophin;    Actinin;    Monoclonal antibody;    Muscular dystrophy;    Recombinant fusion protein;    pATH vector;   

DOI  :  10.1016/0014-5793(90)80460-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A monoclonal antibody, MANDYS141, binds to both dystrophin and actinin on Western blots (SDS-denatured), but only to actinin in frozen sections of human muscle (native conformation). It differs from a polyclonal cross-reacting antiserum in that it binds to several muscle isoforms of actinin (smooth, fast and slow) from man, mouse and chicken and recognises a quite different part of the proposed triple-helical region of dystrophin (amino acids 1750–2248). The results suggest that structural homologies between actinin and dystrophin occur more than once in their central helical regions and provide experimental support for an actinin-like central rod model for dystrophin.

【 授权许可】

Unknown