Identification of some of the brain Gn27 as the ral gene product Comparison between the brain and platelet Gn‐proteins

FEBS Letters

Bhullar, Rajinder P.¹ 

[1] Departments of Oral Biology, and Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Manitoba, Canada

关键词: Gn-protein;    GTP-binding protein;    Gn27;    Brain;    Platelet;    ral;    Gn-proteins;    23–27 kDa proteins detected by binding of [α−32P]GTP to nitrocellulose blots [1];   

DOI  :  10.1016/0014-5793(92)80022-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two major G_n-proteins, G_n27 and G_n26, were detected in the 100,000 × g_av particulate fraction of rabbit and bovine brain. The G_n26 protein was also present in significant amounts (∼50% of total) in the brain supernatant fraction. An antiserum raised against recombinant simian ralA recognized a 27-kDa brain protein with the same apparent molecular mass as the G_n27 protein. In further analysis by two-dimensional polyacrylamide gel electrophoresis, the brain particulate G_n-proteins were resolved into 6 major forms, four of 27 kDa (G_n27a–d) and two of 26 kDa (G_n26a and G_n26b). Minor GTP-binding components were also observed at 25 kDa and 24 kDa. The ratA antibody reacted strongly with the brain G_n27b form and weakly with the G_n27a and G_n27c but not with G_n27d or any of the other G_n-proteins. In addition, comparison of human platelet and bovine brain particulate G_n-proteins by two-dimensional polyacrylamide gel electrophoresis demonstrated a tissue/cell-type specific expression of the various forms of G_n-proteins.

【 授权许可】

Unknown