Identification of multiple ral gene products in human platelets that account for some but not all of the platelet Gn‐proteins

FEBS Letters

Haslam, Richard J.¹  Bhullar, Rajinder P.¹  Chardin, Pierre² 

[1] Department of Pathology, McMaster University, Hamilton, Ontario, Canada L8N 3Z5;INSERM U-248, Faculté de Médicine Lariboisière- Saint Louis, 75010 Paris, France

关键词: Gn-protein;    GTP-binding protein;    Gene product;    ral;    (Platelet);    Gn-proteins;    23-27 kDa proteins detected by binding of [(α-32p]GTP to nitrocellulose blots [1];   

DOI  :  10.1016/0014-5793(90)80063-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyclonal antibodies raised against specific recombinant low molecular mass GTP-binding proteins were tested for their ability to recognize partially purified human platelet membrane G_n-proteins (i.e. proteins that bind [α-³²PlGTP on nitrocellulose blots of SDS/polyacrylamide gels). An antiserum against simian ralA protein recognized a 27 kDa human platelet protein with the same apparent molecular mass as the major platelet G_n-protein (G_n27). In further analysis by two-dimensional polyacrylamide gel electrophoresis, the isoelectric focusing step permitted resolution of 12 major G_n-protein forms, seven of 27 kDa (G_n27a-g), one of 26 kDa (G_n26) and four of 24 kDa (G_n24a-d). The ralA antibody reacted strongly with the five most basic G_n27 species (a-e), weakly with G_n26 and not at all with G_n27f, G_n27g or G_n24a-d. We conclude that ral gene products account for some but probably not for all of the platelet G_n-proteins.

【 授权许可】

Unknown