Isolation of factor Xa from chick embryo as the amniotic endoprotease responsible for paramyxovirus activation

FEBS Letters

Gotoh, Bin¹  Yamauchi, Fumiko²  Ogasawara, Tomohiko³  Nagai, Yoshiyuki² 

[1] Radioisotope Center Medical Division, Nagoya University School of Medicine, Nagoya 466, Japan;Research Institute for Disease Mechanism and Control Nagoya University School of Medicine, Nagoya 466, Japan;1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya 466, Japan

关键词: Virus activating protease;    Factor X;    Viral tropism;    Chick amniotic fluid;    FX;    factor X;    FXa;    activated form of FX;    VAP;    virus activating protease;    CE;    chick embryo;    AMF;    amniotic fluid;    ALF;    allantoic fluid;   

DOI  :  10.1016/0014-5793(92)80303-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In chick embryo, certain paramyxoviruses mainly target the chorioallantois and the allantoamnion and show no extensive further spreading in the other organs. This has been explained by the possible presence of an endoprotease activating the viral fusion glycoprotein precursor in the allantoic and the amniotic fluid, and its absence in other places or organs, We previously isolated such an endoprotease from the allantoic fluid and demonstrated its identity with the clotting factor Xa. Exactly the same endoprotease by all the criteria including the N-terminal amino acid sequence was now isolated from the amniotic fluid. Thus, the factor Xa seems to be a major host determinant of the viral tropism in chick embryo.

【 授权许可】

Unknown