erythro‐β‐hydroxyaspartic acid in bovine factor IX and factor X

FEBS Letters

Stenflo, Johan¹  Sugo, Teruko¹  Fernlund, Per¹ 

[1] Department of Clinical Chemistry, University of Lund, Malmö General Hospital, S-214 01 Malmö, Sweden

关键词: β-Hydroxyaspartic acid;    Posttranslational modification;    Factor IX;    Factor X;    Vitamin K-dependent protein;    Blood coagulation;   

DOI  :  10.1016/0014-5793(84)80023-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To localize β-hydroxyaspartic acid in factor IX and factor X the two proteins were cleaved with cyanogen bromide and trypsin, respectively. Peptides containing β-hydroxyaspartic acid were isolated and subjected to Edman degradation. The phenylthiohydantoin derivative of β-hydroxyaspartic acid was identified by HPLC in position 3 in the factor IX fragment and in position 1 in the factor X fragment. This corresponds to position 64 in factor IX and position 63 in the light chain of factor X. The assignments were confirmed by subtractive Edman degradation and with the dansyl method.

【 授权许可】

Unknown