Primary structure of the virus activating protease: from chick embryo Its identity with the blood clotting factor Xa

FEBS Letters

Suzuki, Harukazu²  Harada, Atzuko²  Gotoh, Bin¹  Wada, Kohji²  Asaka, Jun-ichiro²  Ogasawara, Tomohiko¹  Nagai, Yoshiyuki¹  Hayashi, Yumiko² 

[1] Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466, Japan;Shianogi Institute for Medical Science, Settsu-shi, Osaka 566, Japan

关键词: Virus activating protease;    Factor X;    Stuart factor;    Chick embryo;    FX;    factor X;    FXa;    activated form of FX;    VAP;    virus activating protease;    CAM;    chorioallantoic membrane;   

DOI  :  10.1016/0014-5793(91)80608-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Host cell proteases activating, para- and orthomyxovirus fusion glycoprotein precursors play a crucial role in determining the viral tropism in infected organisms, We previously isolated such an endoprotease from the allantoic fluid of chick embryo and showed its close similarity to the activated form of blood clotting factor X (FXa) by partial amino acid sequencing. In this report, we have cloned and sequenced a cDNA of the protease and show that it is encoded in a single gene as a preproform with all the functional and structural domains known to be characteristic of bovine or human FX, establishing the identity between the protease and FXa.

【 授权许可】

Unknown