Identification of different receptor types for toxic phospholipases A2 in rabbit skeletal muscle

FEBS Letters

Lazdunski, Michel¹  Barhanin, Jacques¹  Lambeau, Gérard¹ 

[1] Institut de Pharmacologie Moléculaire et Cellulaire, 660 route des Lucioles, Sophia Antipolis 06560 Valbonne, France

关键词: Skeletal muscle;    Neurotoxin;    Myotoxin;    Phospholipase A2;    Snake venom;   

DOI  :  10.1016/0014-5793(91)81145-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oxyuranus scutellatus scutellatus toxins I (OS₁) and 2 (OS₂) are two phospholipase A₂s (PLA₂) isolated from the venom of the Australian Taipan snake. Their iodinated derivatives have been used to characterize PLA₂ binding sites on rabbit skeletal muscle. Competition and cross-linking experiments indicate that ¹²⁵I-labelled OS₂ binding sites in rabbit skeletal muscle in vivo are distributed into two classes of receptors. One class binds OS₂ and OS₁ and is insensitive to the bee venom PLA₂. It is composed of a 180 kDa binding protein. This class of PLA₂ receptor is expressed at a high level in rabbit myotube membranes. The other class of PLA₂ receptor identified with ¹²⁵I-OS₂ also binds with high affinity the bee venom PLA₂ but not OS₁ and is composed of major polypeptides of 34.48 and 82 kDa. This second class of receptor is similar to the one found in brain membranes. The density of the two classes of receptors varies during muscle development.

【 授权许可】

Unknown