Characterization of multiple nicotinic acetylcholine receptor‐binding proteins and phospholipases A2 from the venom of the coral snake Micrurus nigrocinctus nigrocinctus

FEBS Letters

Thelestam, Monica⁵  Girón-Cortes, Mauricio⁴  Alape-Girón, Alberto¹  Bergman, Tomas²  Stiles, Bradley³  Schmidt, James³  Jörnvall, Hans² 

[1]Departamento de Bioquímica, Facultad de Medicina, Universidad de Costa Rica, San José, Costa Rica

[2]Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden

[3]Toxinology Division, USAMRIID, Frederick, MD, USA

[4]Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San José, Costa Rica

[5]Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden

关键词: Phospholipase A2;    Neurotoxin;    HPLC;    Monoclonal antibody;    Amino acid sequence;    Micrurus;   

DOI  :  10.1016/0014-5793(95)01543-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The presence of multiple nicotinic acetylcholine receptor (AchR)-binding proteins and phospholipases A₂ was detected in the venom of a member of the Elapinae subfamily, Micrurus nigrocinctus nigrocinctus. Multi-step chromatographies were used to isolate four AchR-binding proteins (Mnn-9, Mnn-4, Mnn-3C and Mnn-1A) and five basic PLA₂s (nigroxins A, B, C1, C2 and C3). The Micrurus AchR-binding proteins are antigenically and structurally related to short- and long-chain α-neurotoxins from Naja. The nigroxins are antigenically similar and constitute a new antigenic subclass of PLA₂s. Nigroxins A and B are class I PLA₂s, structurally more related to enzymes from Bungarinae than to those from Hydrophinae/Laticaudinae. These data contribute to clarify the relationships between Micrurus venom proteins and other elapid toxins and may be useful to improve the neutralizing efficiency of antivenoms.

【 授权许可】

Unknown