A novel brain‐specific 25 kDa protein (p25) is phosphorylated by a Ser/Thr‐Pro kinase (TPK II) from tau protein kinase fractions

FEBS Letters

Omori, Akira¹  Sato, Showbu¹  Takahashi, Miho¹  Sato, Kazuki¹  Imahori, Kazutomo¹  Uchida, Tsuneko¹  Tomizawa, Kayoko¹  Shiratsuchi, Akiko¹  Ishiguro, Koichi¹ 

[1] Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan

关键词: Brain-specific protein;    Phosphorylation;    Protein kinase;    Immunoblot analysis;    PBS;    phosphate-buffered saline;    MTPs;    microtubule-associated proteins;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(91)80903-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel brain-specific 25 kDa protein (p25) was purified from a bovine brain extract. The protein was phosphorylated by Ser/Thr-Pro kinase (TPK II) in tau protein kinase fractions at the Ser residues of Ser-Pro sequences. Using immunoblot analysis, the protein was found only in brain extracts, and was most abundant in the brain regions such as cerebrum and hippocampus, but less abundant in cerebellum, medulla oblongata and olfactory bulb. The protein was detected in rat, bovine and human brain extracts, indicating that this protein specifically exists in mammalian brain tissues.

【 授权许可】

Unknown