Differences between embryonic and adult Torpedo acetylcholine receptor γ subunit

FEBS Letters

Carmon, Sara¹  Fuchs, Sara¹  Safran, Anat¹  Souroujon, Miriam C.¹ 

[1] Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: Adult and embryonic acetylcholine receptor;    γ Subunit;    Anti-peptide antibody;    Phosphorylation;   

DOI  :  10.1016/0014-5793(91)81039-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies to a synthetic peptide corresponding to residues 346–359 of the Torpedo acetylcholine receptor (AChR) γ subunit, were employed to compare the adult and embryonic receptor. This peptide contains a consensus phosphorylation site for cAMP-dependent protein kinase (PKA). The anti-peptide antibodies discriminated between adult and embryonic AChRs, and reacted preferentially with the adult γ form. These observed immunological differences did not seem to stem from different phosphorylation states. Our results suggest that the embryonic Torpedo AChR may have a γ-like subunit that differs from the known adult form of this subunit, at least in the specific region that contains the phosphorylation site for PKA.

【 授权许可】

Unknown