Oxidized low density lipoprotein inhibits bradykinin‐induced phosphoinositide hydrolysis in cultured bovine aortic endothelial cells

FEBS Letters

Hirata, Ken-ichi¹  Akita, Hozuka¹  Yokoyama, Mitsuhiro¹ 

[1] The First Department of Internal Medicine, Kobe University School of Medicine, Kobe 650, Japan

关键词: Endothelium;    Bradykinin;    Endothelium-derived relaxing factor;    Intracellular calcium;    Low-density lipoprotein;   

DOI  :  10.1016/0014-5793(91)80046-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vascular endothelial cells, in response to various neurohumoral and physical stimuli, produce an endothelium-derived relaxing factor, a substance which regulates vascular tone. We have demonstrated that oxidized low density lipoprotein (LDL) inhibits endothelium-dependent relaxation. We studied the effect of oxidized LDL on inositol phosphates formation stimulated with bradykinin (BK) in cultured bovine aortic endothelial cells. BK elicited a rapid generation of inositol phosphates from inositol phospholipids. Accumulation of inositol 1,4,5-trisphosphate (IP3) stimulated with BK (0.1 μM) was markedly inhibited by oxidized LDL. However, native LDL had little effect on BK-induced accumulation of IP3. From these results, oxidized LDL inhibits receptor-mediated phosphoinositides hydrolysis and modulates the endothelial function.

【 授权许可】

Unknown