Molecular cloning and characterization of human endothelial nitric oxide synthase

FEBS Letters

Chen, Hai Sheine¹  Lamas, Santiago⁴  Marsden, Philip A.³  Sundell, Cynthia L.²  Michel, Thomas⁴  Schappert, Keith T.¹  Wilcox, Josiah N.²  Flowers, Michele³ 

[1] Department of Genetics, Hospital for Sick Children, University of Toronto, Toronto, Ont., Canada;Department of Medicine, Emory University, Atlanta, GA, USA;Renal Division and Department of Medicine, St. Michael's Hospital, University of Toronto, Toronto, Ont., Canada;Cardiovascular Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA

关键词: Cyclic GMP;    Endothelium-derived relaxing factor;    Endothelium;    Vascular smooth muscle;    Vasodilatation;    HUVEC;    human umbilical vein endothelial cells;    kb;    kilobases;    PCR;    polymerase chain reaction;    TNF-α;    tumor necrosis factor-alpha;   

DOI  :  10.1016/0014-5793(92)80697-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The constitutive calcium/calmodulin-dependent nitric oxide (NO) synthase expressed in vascular endothelium shares common biochemical and pharmacologic properties with neuronal NO synthase. However, recent cloning and molecular characterization of NO synthase from bovine endothelial cells indicated the existence of a family of constitutive NO synthases. Accordingly, we undertook molecular cloning and sequence analysis of human endothelial NO synthase. Complementary DNA clones predict a protein of 1,203 amino acids sharing 94% identity with the bovine endothelial protein, but only 60% identity with the rat brain NO synthase isoform. Northern blot analysis with an endothelial-derived cDNA identified a 4.6–4.8 kb mRNA transcript in HUVEC and in situ hybridization localized transcripts to vascular endothelium but not neuronal tissue.

【 授权许可】

Unknown