Confocal microscope is able to detect calcium metabolic in neuronal infection by toxoplasma gondii

International Conference on Physical Instrumentation and Advanced Materials

物理学;材料科学

Sensusiati, A.D.^1 ; Priya, T.K.S.^1 ; Dachlan, Y.P.^1

Medical Faculty of Airlangga University, Dr. Soetomo Hospital, Surabaya, Indonesia^1

关键词: Calcium metabolism;    Correlation tests;    Detection of changes;    Fluorescent emission;    Intracellular calcium;    Kolmogorov-Smirnov test;    Pearson correlation;    Quantitative and qualitative methods;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/853/1/012015/pdf
DOI  :  10.1088/1742-6596/853/1/012015

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Calcium metabolism plays a very important role in neurons infected by Toxoplasma. Detection of change of calcium metabolism of neuron infected by Toxoplasma and Toxoplasma requires the calculation both quantitative and qualitative method. Confocal microscope has the ability to capture the wave of the fluorescent emission of the fluorescent dyes used in the measurement of cell calcium. The purpose of this study was to prove the difference in calcium changes between infected and uninfected neurons using confocal microscopy. Neuronal culture of human-skin-derived neural stem cell were divided into 6 groups, consisting 3 uninfected groups and 3 infected groups. Among the 3 groups were 2 hours, 24 hours and 48 hours. The neuron Toxoplasma gondii ratio was 1:5. Observation of intracellular calcium of neuron and tachyzoite, evidence of necrosis, apoptosis and the expression of Hsp 70 of neuron were examined by confocal microscope. The normality of the data was analysed by Kolmogorov-Smirnov Test, differentiation test was checked by t2 Test, and ANOVAs, for correlation test was done by Pearson Correlation Test. The calcium intensity of cytosolic neuron and T. gondii was significantly different from control groups (p