Magnetic resonance studies of murine macrophages

FEBS Letters

Ward, Mary H.³  King, Nicholas J.C.²  Holmes, Kerry T.¹ 

[1] Department of Cancer Medicine, University of Sydney, N.S.W. 2006, Australia;Department of Anatomy, University of Sydney, N.S.W. 2006, Australia;Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia

关键词: NMR;    1H-;    Macrophage;    IFN-γ;    Activation;    1H-MRS;    proton magnetic resonance spectroscopy;    TSB;    Trypticase Soy Broth;    PBS;    phosphate-buffered saline;    SCC;    standard culture conditions;    MEM;    Eagle's minimal essential medium;    FCS;    foetal calf serum;    CRBC;    chick red cells;   

DOI  :  10.1016/0014-5793(91)80024-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proton magnetic resonance spectroscopy (¹H-MRS) was used to investigate the membranes of macrophages activated by γ-interferon in vitro and by Listeria monocytogenes in vivo. We report the appearance with activation, of a high resolution spectrum indistinguishable from that found in activated T and B cells and embryonic and malignant cell types previously studied. We furthermore show that proliferation is not a prerequisite for the appearance of this activated spectrum. This supports the idea that membrane ‘activation’ in all cells, irrespective of origin, may be accompanied by similar architectural changes, and suggests that a common pathway exists for the activation of cell membranes of the immune system, possibly important in the acquisition of increased motility. The use of ¹H-MRS as a non-invasive tool for analysis of activation is discussed.

【 授权许可】

Unknown