Complete down‐regulation of low‐density lipoprotein receptor activity in human liver parenchymal cells by β‐very‐low‐density lipoprotein

FEBS Letters

Kuiper, Johan¹  van Berkel, Theo J.C.¹  Kamps, Jan A.A.M.¹  Kar Kruijt, J.¹ 

[1] Division of Biopharmaceutics, Center for Bio-Pharmaceutical Sciences, Sylvius Laboratories, University of Leiden, P.O. Box 9503, 2300 RA Leiden, The Netherlands

关键词: Low-density lipoprotein;    β-very-low-density lipoprotein;    LDL-receptor: Human parenchymal liver cell: Receptor-regulation;    LDL;    low-density lipoprotein;    VLDL;    very-lowdensity lipoprotein;    β-VLDL;    cholesteryl ester-rich β-migrating very-low-density lipoprotein;   

DOI  :  10.1016/0014-5793(91)80010-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of LDL and β-VLDL on the expression of the LDL receptor is studied in cultured human parenchymal cells. The high affinity binding of [¹²⁵I]LDL to cultured human parenchymal cells was down regulated to 37.3±2.9% and 24.0±2.6% of the control value, after preincubation with LDL or β-VLDL for 22 h, respectively. When LDL receptor synthesis was blocked at 22 h a residual receptor activity of 29% is noticed, indicating a half-life of LDL receptors in human parenchymal cells of 12 h. It is concluded that LDL receptor expression on human liver parenchymal cells is subject to complete down-regulation by β-VLDL, which may be held responsible for the cholesterol-rich diet induced down-regulation of LDL receptors, in vivo.

【 授权许可】

Unknown