Pyrrolidine dithiocarbamate is a potent antioxidant against hypochlorous acid‐induced protein damage

FEBS Letters

Zhu, Ben-Zhan¹  Frei, Balz¹  Carr, Anitra C¹ 

[1] Linus Pauling Institute, Oregon State University, 571 Weniger Hall, Corvallis, OR 97331-6512, USA

关键词: Pyrrolidine dithiocarbamate;    Hypochlorous acid;    α1-Antiproteinase;    Protein carbonyl;    Low-density lipoprotein;    apoB;    apolipoprotein B-100;    BSA;    bovine serum albumin;    DDTC;    diethyldithiocarbamate;    DNPH;    2;    4-dinitrophenylhydrazine;    GSH;    reduced glutathione;    HOCl;    hypochlorous acid;    IC50;    concentration required for 50% inhibition;    LDL;    low-density lipoprotein;    NAC;    N-acetylcysteine;    PDTC;    pyrrolidine dithiocarbamate;    REM;    relative electrophoretic mobility;   

DOI  :  10.1016/S0014-5793(02)03637-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The antioxidant potential of the dithiol compound pyrrolidine dithiocarbamate (PDTC) against protein damage induced by hypochlorous acid (HOCl) was investigated. The effects of PDTC were compared to those of reduced glutathione (GSH) and N-acetylcysteine (NAC). PDTC markedly and in a concentration-dependent manner inhibited HOCl-induced inactivation of α₁-antiproteinase, protein carbonyl formation on serum albumin and oxidation of human low-density lipoprotein. The direct scavenging of HOCl by PDTC was demonstrated by two quantitative methods, oxidation of ferrocyanide and chlorination of monochlorodimedon. In all assay systems, PDTC was two to three times more potent than GSH and NAC, while diethyldithiocarbamate was about as effective as PDTC. These data demonstrate that PDTC is a potent antioxidant against HOCl-induced protein oxidative damage, suggesting that PDTC might be useful in the prevention and treatment of inflammatory conditions.

【 授权许可】

Unknown