Involvement of endotoxins or tumor necrosis factor‐α in macrophage‐mediated oxidation of low density lipoprotein

FEBS Letters

Fujiwara, Kyoko¹  Bannai, Shiro¹  Sato, Hideyo¹ 

[1] Department of Biochemistry, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan

关键词: Low-density lipoprotein;    Macrophage;    Lipopolysaccharide;    Tumor necrosis factor-α;    Cystine;    LDL;    low density lipoprotein;    LPS;    lipopolysaccharide;    TNF-α;    tumor necrosis factor-α;    IL-1β;    interleukin-1β;    HEPES;    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid;    MEM-HEPES(−)PR;    phenol red-free minimum essential medium buffered with 10 mM HEPES;    TBARS;    thiobarbituric acid-reactive substances;   

DOI  :  10.1016/S0014-5793(98)00735-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oxidation of low-density lipoprotein (LDL) can be mediated in vitro by cultured cells, including macrophages. This cellular oxidation is dependent on the production of free thiols by the cells in the presence of transition metal ions. We now report that the production of thiols by macrophages is greatly enhanced when cells are cultured with lipopolysaccharide (LPS) or tumor necrosis factor-α (TNF-α). Oxidation of LDL by macrophages is markedly augmented by pre-treatment of the cells with LPS or TNF-α. The results suggest that activation by endotoxins or TNF-α is a necessary step for macrophages to mediate oxidation of LDL.

【 授权许可】

Unknown