Structural requirements for oxidation of low‐density lipoprotein by thiols

FEBS Letters

Graham, Annette¹  Wood, Jenny L.¹ 

[1] Biology Division, Wellcome Research Laboratories, Biology Division, Langley Court, Beckenham, Kent BR3 3BS, UK

关键词: Atherosclerosis;    Oxidized LDL;    Macrophage;    Smooth muscle cell;    Thiol;    Superoxide;    Low-density lipoprotein;    (LDL);    ethylene diamine tetraacetic acid;    (EDTA);    butylated hydroxytoluene;    (BHT);    relative electrophoretic mobility;    (REM);    superoxide dismutase;    (SOD);    heat-inactivated superoxide dismutase;    (HI-SOD);   

DOI  :  10.1016/0014-5793(95)00494-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oxidation of low-density lipoprotein (LDL) by macrophages, endothelial cells and smooth muscle cells, may be mediated by production of free thiols in the presence of transition metals. We examined the structural requirements, within a series of cysteinyl derivatives, for oxidation of thiols and of LDL in Hams F10 medium. The primary mechanism by which such thiols mediate oxidation of LDL is largely independent of superoxide production, but strongly correlated with the susceptibility of each thiol to iron-catalysed auto-oxidation. These effects are compared and contrasted with thiol-dependent oxidation of LDL by stimulated human monocytes and macrophages.

【 授权许可】

Unknown