Reconstitution of active sucrose transport in plant proteoliposomes

FEBS Letters

Gallet, Olivier¹  Lemoine, Rémi¹  Delrot, Serge¹  Gaillard, Cécile¹  Li, Ze-Sheng¹ 

[1]Laboratoire de Physiologie et Biochimie Végétales, UA CNRS 574, University of Poitiers, 25 rue de Faubourg Saint-Cyprien, 86000 Poitiers, France

关键词: Sugar transport;    Reconstitution;    Sucrose;    Plant plasma membrane;    Beta vulgaris L;    CHAPS;    3-[(3-cholamidopropyl)-dimethyl-ammonio]-1-propane sulfonate;    DTT;    dithiothreitol;    NEM;    N-ethylmaleimide;   

DOI  :  10.1016/0014-5793(91)80954-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The proteins of purified plasma membranes from sugar beet (Beta vulgaris L.) leaf were solubilized and separated on a size exclusion column. The fractions eluted from the column were monitored by ELISA with antibodies directed to a putative sucrose carrier protein. The peak most reactive in ELISA was approximately 120 kDa, and yielded a 40 kDa peak after denaturation by SDS. The 120-kDa peak was recovered and used for reconstitution experiments with asolectin. Upon imposition of an artificial pH gradient and electrical gradient, the obtained proteoliposomes exhibited active transport of sucrose, but not of valine. The active transport of sucrose was inhibited by N-ethylmaleimide and HgCl₂.

【 授权许可】

Unknown