Lysophosphatidylcholine specifically stimulates plasma membrane H+‐ATPase from corn roots

FEBS Letters

Nasirova, G.F.¹  Palladina, T.A.¹  Pedchenko, V.K.¹ 

[1]Institute of Plant Physiology and Genetics, Ukrainian Academy of Sciences, Kiev, USSR

关键词: Lysophosphatidyl choline;    Enzyme activation;    H+-ATPase;    Plant plasma membrane;    lysoPC;    lysophosphatidylcholine;    PMSF;    phenylmethylsulfonyl fluoride;    MES;    morpholynoethane sulfonic acid;    zwittergent 3–14;    N-tetradecyl-N-N-dimethyl-3-ammonia-1-propane-sulfonate;    DTT;    dithiothreitol;    SDS;    sodium dodecylsulfate;    TX-100;    Triton X-100;    p-NPP;    4-nitrophenyl phosphate;    p-NP;    4-nitrophenol;    CMC;    critical micellar concentration;   

DOI  :  10.1016/0014-5793(90)81472-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The plasma membrane H⁺-ATPase activity from corn seedling roots is shown to be stimulated 3- to 4-fold by the addition of lysophosphatidylcholine (lysoPC). This effect clearly differs from that of other detergents by both the magnitude and the absence of inhibition at higher concentrations. LysoPC decreases the apparent K _m for MgATP, increases V _max of the ATPase reaction but does not change its pH optimum. On the contrary, the acid phosphatase activity associated with plasma membranes is not influenced by lysoPC. A lysoPC stimulation is also demonstrated for the solubilized preparation of the H⁺-ATPase. It is assumed that lysoPC stimulation of the plant plasma membrane H⁺-ATPase is not only due to permeabilization of the vesicles for MgATP, but also direct action on the enzyme.

【 授权许可】

Unknown