Proton‐motive‐force‐driven sucrose uptake in sugar beet plasma membrane vesicles

FEBS Letters

Lemoine, Rémi¹  Delrot, Serge¹ 

[1]Laboratoire de Physiologie et Biochimie Végétales, UA CNRS 574, University of Poitiers, 25 rue du Faubourg Saint-Cyprien, 86000 Poitiers, France

关键词: Sugar transport;    Sucrose;    Protonmotive force;    Plasma membrane vesicle;    (Beta vulgaris L.);    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    DTT;    dithiothreitol;    NEM;    N-ethylmaleimide;   

DOI  :  10.1016/0014-5793(89)80030-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Highly purified plasma membranes were prepared by aqueous two-phase partioning from sugar beet (Beta vulgaris L.) leaf microsomes. Uptake of sucrose was studied with non-energized vesicles and with vesicles artificially energized by a pH gradient (ΔpH) or a pH gradient plus an electrical gradient (ΔpH+Δψ). In non-energized vesicles, sucrose was taken up via a diffusion-like system until attainment of concentration equilibrium. Energized vesicles rapidly accumulated sucrose with accumulation ratios of 6 and 12 (vs the external concentration) for vesicles energized by ΔpH and ΔpH + Δψ, respectively. Energized uptake exhibited saturation kinetics, and was sensitive to carbonyl cyanide m-chlorophenylhydrazone and N-ethylmaleimide.

【 授权许可】

Unknown